As the name centrifuge suggests, these machines use centrifugal force to separate substances. Serious injuries or potential exposures can occur if centrifuges are improperly used or maintained. A. Contact a qualified service technician if inspection identifies centrifuge components requiring repair or replacement. The Centers for Disease Control and Prevention (CDC) cannot attest to the accuracy of a non-federal website. Avoid abrasive wire brushes for cleaning. ☐ YES ☒ NO, Laboratory Director - Dave Bridges Revision Date - 2016-10-14, http://bridgeslab.sph.umich.edu/protocols/index.php?title=SOP_-_Centrifuge_Safety&oldid=1566. Balance centrifuge: Follow manufacturer’s instructions for proper centrifuge balancing steps. Centrifuges are machines used to separate solids from liquids in a suspension. Avoid alkaline cleaners for aluminum centrifuge components. Mon-Fri 8 am – 4:30 pm, Sat 9 am – 12 pm At the same time, objects that are less dense are displaced and move to the centre. If a specific procedure does not allow for this, contact Occupational Safety and Environmental Health (OSEH) at (734) 647-1143 for guidance. 1. Other safety devices include safety centrifuges with automatic locking mechanisms or solid lids, safety centrifuge cups, safety blenders, safety sonicators. For biohazardous materials, clean/disinfect centrifuge and remove/cross out biohazard sticker. Mon-Fri 7:30 am - 4:30 pm The centrifuge must be loaded carefully balanced. The following information may be integrated into a lab-specific standard operating procedure (SOP) for centrifuge use. Maintain log book: For all high speed centrifuges and ultracentrifuges include run dates, durations, speeds, total rotor revolutions, and notes on rotor condition. If centrifuging hazardous materials, care should be taken to use tightly capped tubes and/or sealable safety cups or rotors that can be loaded and unloaded in a fume hood or biosafety cabinet, depending on the hazard. Contact for current hours as they may vary Centrifuges are instruments used to separate mixtures, based on particle size and density, by spinning the mixtures at high speeds. To receive email updates about this page, enter your email address: Fundamentals of Chemical Fume Hood Safety, Fundamentals of Working Safely in a Biological Safety Cabinet, Centers for Disease Control and Prevention. Note: Rotor life span may be reduced or warranty voided if autoclaved; contact manufacturer for guidance. After hours - go to UM Hospital Emergency Dept. This basic-level eLearning course provides information on the safe use of centrifuges. Public health and clinical laboratory staff, safety professionals and persons interested in safe use of centrifuges. Sign by logging in and typing * ~~~~ in the list below: Prior Approval required – Is this procedure hazardous enough to warrant prior approval from the Laboratory Director? In a laboratory centrifuge that uses sample tubes, the radia… This means that lab managers can improve centrifuge safety at their organizations by refining operating and maintenance procedures and emphasizing staff training. Reference centrifuge operator’s manual or contact manufacturer for guidance. TREATMENT FACILITIES: Follow manufacturer’s filling limits for tubes. Ensure gloves are compatible with hazard(s). A centrifuge is a device that uses centrifugal force to separate component parts of a liquid or fluid (and even gases). – Urgent Care Clinic The Charles Woodson Children's Clinical Research Lab is accessible 24/7. Open centrifuge tubes that contain biohazardous specimens inside a BSC with the tube pointed away from you. C. Open sealable tubes/safety cups/rotors: Wear appropriate PPE and open inside fume hood or biosafety cabinet, depending on hazard. Use a vacuum system with appropriate in-line reservoirs and filters. 207 Fletcher Street, Ann Arbor (734) 764-8320 Report all emergencies, suspicious activity, injuries, spills, and fires to the University of Michigan Division of Public Safety and Security (DPSS) by calling 911 or texting 377911. C. Inspect centrifuge: Ensure tubes are rated for intended use (speed, temperature, and chemical resistance). Stop run: Ensure centrifuge comes to complete stop before opening cover. For chemical, radioactive, and biohazard spills, contact OSEH at (734) 647-1143 for assistance and guidance. This course is approved for 0.5 contact hours. The Charles Woodson Children's Clinical Research Laboratory requires that all users have undergone appropriate OSEH or equivalent training prior to gaining access to the lab (See detailed instructions in the Woodson Lab Core section). Complete lab-specific training for the centrifuge. Equipment repair and adjustments shall only be conducted by qualified service technicians. P.A.C.E.® course number: 288-001-20. Furthermore, all personnel shall read and fully adhere to this SOP when handling centrifuges. Contact OSEH for advice on symptoms of chemical exposure, or assistance in performing an exposure assessment. When centrifuging hazardous materials, use tightly capped tubes and/or sealable safety cups or rotors that can be loaded and unloaded in a fume hood or biosafety cabinet, depending on hazard. Ensure rotor is compatible with centrifuge and seated on the drive correctly. Register with the University of Michigan Emergency Alert System via Wolverine Access. cream from milk) or liquids from solids. 1500 East Medical Center Drive, Ann Arbor, (734) 936-6666 Do not decant or pour off supernatant. UMHS Emergency Department -- after clinic hours or on weekends Complete lab-specific training for the centrifuge. Turn off centrifuge immediately and unplug power cord. University Health Services -- University students (non-life threatening conditions) A. U-M Occupational Health Services -- Campus Employees I agree to contact my Supervisor or Lab manager if I plan to modify this procedure. When operating the centrifuge, proper eye protection (glasses/goggles), gloves, lab coat, and close-toe footwear should be worn. CDC twenty four seven. A certified biological safety cabinet (class I or II) is the primary barrier to protect worker from aerosols if working with RG2 or higher agents. Check for leaks/spills: In samples, rotor, safety cups/buckets, and centrifuge well. Confirm rotor is balanced. Use tongs or forceps to avoid contact with sharp-edged debris.

laboratory centrifuge training

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